Recombinant Anti-TMEM16A/DOG1 antibody (Rabbit mAb)
   

Recombinant Anti-TMEM16A/DOG1 antibody (Rabbit mAb)

Antibodies-Oncology
AO-03-GB15920-50
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Product Information

Protein full name

Anoctamin-1

Synonyms

Ano1, ANO1, DOG1, ORAOV2, TAOS2, TMEM16A, Anoctamin 1

Immunogen

Recombinant protein corresponding to Human TMEM16A

Uniprot ID

Q5XXA6

Isotype

IgG

Clone Number

SB659

Purity

Affinity purification

Subcellular location

Apical cell membrane, Presynapse

Applications

Species

Dilution

Positive Tissue

IHC/IF

Human

1: 1000-1: 2000

esophageal cancer, ovarian cancer, lung

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Anoctamin-1 (ANO1) also known as Transmembrane member 16A (TMEM16A) is a protein encoded by the ANO1 gene. Anoctamin-1 is a voltage-sensitive calcium-activated chloride channel that is expressed in smooth muscle and epithelial cells; it is highly expressed in human interstitial cells of Cajal (ICC) throughout the gastrointestinal tract. Changes in ANO1 channel activity directly/positively correlate with ICC activity.

Images

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IHC analysis of TMEM16A/DOG1(GB15920).
Sample: Human esophageal carcinoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Antigen retrieval instrument: (ARI-4),Tris-EDTA buffer (pH 9.0) (G1203), Water bath 100℃ 25min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

IHC analysis of TMEM16A/DOG1(GB15920).
Sample: Human lung, 4% PFA (G1101) 12-24h.
Antigen retrieval: Antigen retrieval instrument: (ARI-4),Tris-EDTA buffer (pH 9.0) (G1203), Water bath 100℃ 25min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

IF analysis of TMEM16A/DOG1(GB15920)red.
Sample: Human ovarian cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Antigen retrieval instrument: (ARI-4),Tris-EDTA buffer (pH 9.0) (G1203), Water bath 100℃ 25min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

For Research Use Only!                                               Ver. No.: V2.0-20251230


Antibodies-Oncology
: AO-03-GB15920-50
: 10 Items
Hurry! only 10 items left in stock.

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