Recombinant Anti-S100A8 antibody (Rabbit mAb)

Protein full name

Protein S100-A8

Synonyms

S100A8, 60B8AG, CAGA, CFAG, CGLA, CP-10, L1Ag, MA387, MIF, MRP8, NIF, P8, S100 calcium

Immunogen

Recombinant protein corresponding to Human S100A8

Uniprot ID

P05109

Isotype

IgG

Clone Number

SB672

Purity

Affinity purification

Predicted MW./Observed MW.

11 kDa / 11 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human

1: 500-1: 1000

THP-1, HL-60, SK-BR-3

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase.

Images

WB analysis of S100A8(GB150104).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.