Recombinant Anti-ADAMTS4 antibody (Rabbit mAb)

Synonyms

Adamts4, ADAMTS4, ADAMTS-2, ADAMTS-4, ADMP-1, ADAM metallopeptidase with thrombospondin type 1 motif 4, ATS4, ADAM-TS 4, ADAM-TS4, ADAMTS-4

Immunogen

Recombinant protein corresponding to Human ADAMTS4

Uniprot ID

O75173

Isotype

IgG

Clone Number

SB532

Purity

Affinity purification

Subcellular location

Secreted

Predicted MW./Observed MW.

90 kDa / 90 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human

1: 1000-1: 4000

SH-SY5Y, SW1353, U-87 MG

IHC/IF

Human

1: 1000-1: 2500

breast cancer, fibrotic lung, glioblastoma multiforme

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

A disintegrin and metalloproteinase with thrombospondin motifs 4 is an enzyme encoded by the ADAMTS4 gene. ADAMTS4 is the shortest known ADAMTS, lacking the C-terminal domain and is the only non-glycosylated ADAMTS. This protein cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. The protein may play an important role in the destruction of aggrecan in arthritic diseases. It could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease.

Images

WB analysis of ADAMTS4(GB15807).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

IHC analysis of ADAMTS4(GB15807).
Sample: Human breast cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Antigen retrieval instrument: (ARI-4),Tris-EDTA buffer (pH 9.0) (G1203), Water bath 100℃ 25min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

IF analysis of ADAMTS4(GB15807)red.
Sample: Human glioblastoma multiforme, 4% PFA (G1101) 12-24h.
Antigen retrieval: Antigen retrieval instrument: (ARI-4),Tris-EDTA buffer (pH 9.0) (G1203), Water bath 100℃ 25min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.