Recombinant Anti-ADAMTS5 antibody (Rabbit mAb)

Protein full name

A disintegrin and metalloproteinase with thrombospondin motifs 5

Synonyms

ADAMTS11, ADMP2, A disintegrin and metalloproteinase with thrombospondin motifs 5, Aggrecanase-2, ADMP-2, ADAM TS 5, ADAM TS5, ADAMTS 11, ATS5, ADAM-TS 5, ADAM-TS5, ADAMTS-5, ADAMTS5

Immunogen

Recombinant protein corresponding to Human ADAMTS5

Uniprot ID

Q9UNA0

Isotype

IgG

Clone Number

SB530

Purity

Affinity purification

Subcellular location

Secreted

Predicted MW./Observed MW.

102 kDa / 75 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 2000

PC-12, SW1353, placenta

IHC/IF

Human

1: 500-1: 1500

glioblastoma multiforme, breast cancer

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Metalloproteinase that plays an important role in connective tissue organization, development, inflammation and cell migration. Extracellular matrix (ECM) degrading enzyme that show proteolytic activity toward the hyalectan group of chondroitin sulfate proteoglycans (CSPGs) including ACAN, VCAN, BCAN and NCAN.Cleavage within the hyalectans occurs at Glu-Xaa recognition motifs. Plays a role in embryonic development, including limb and cardiac morphogenesis, and skeletal muscle development through its VCAN remodeling properties. Cleaves VCAN in the pericellular matrix surrounding myoblasts, facilitating myoblast contact and fusion which is required for skeletal muscle development and regeneration.Participates in development of brown adipose tissue and browning of white adipose tissue.Plays an important role for T-lymphocyte migration from draining lymph nodes following viral infection.

Images

WB analysis of ADAMTS5(GB150099).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

IHC analysis of ADAMTS5(GB150099).
Sample: Human glioblastoma multiforme, 4% PFA (G1101) 12-24h.
Antigen retrieval: Antigen retrieval instrument: (ARI-4),Tris-EDTA buffer (pH 9.0) (G1203), Water bath 100℃ 25min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

IF analysis of ADAMTS5(GB150099)red.
Sample: Human breast cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Antigen retrieval instrument: (ARI-4),Tris-EDTA buffer (pH 9.0) (G1203), Water bath 100℃ 25min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.