Recombinant Anti-Placental alkaline phosphatase (PLAP) antibody (Rabbit mAb)

Protein full name

Alkaline phosphatase, placental type

Synonyms

Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1, ALPP, PLAP, DOA1, PLA2P, PLAA, PLAP, Glycerophosphatase

Immunogen

Human recombinant PLAP protein expressed in HEK293 cells

Uniprot ID

P05187

Isotype

IgG

Clone Number

SB507

Purity

Affinity purification

Subcellular location

Cell membrane

Predicted MW. / Observed MW.

58 kDa / 68 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human

1: 500-1: 1000

HepG2

IHC/IF

Human

1: 1000-1: 2000

placenta, Seminoma

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Alkaline phosphatase that can hydrolyze various phosphate compounds.

Images

WB analysis of Placental alkaline phosphatase (PLAP)(GB152546).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

IHC analysis of Placental alkaline phosphatase (PLAP)(GB152546).
Sample: Human Seminoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

IF analysis of Placental alkaline phosphatase (PLAP)(GB152546)red.
Sample: Human Placenta, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.