Recombinant Anti-S100A10 antibody (Rabbit mAb)

Protein full name

Protein S100-A10

Synonyms

ANX2LG, CAL1L, CLP11, S100a10, S100 calcium-binding protein A10, Calpactin I light chain, Calpactin-1 light chain

Immunogen

KLH conjugated Synthetic peptide corresponding to Human S100A10

Uniprot ID

P60903

Isotype

IgG

Clone Number

SB475

Purity

Affinity purification

Subcellular location

cell surface, Cytoplasm, extracellular matrix, nuclear matrix, plasma membrane, plasma membrane protein complex

Applications

Species

Dilution

Positive Tissue

IHC/IF

Human, Mouse, Rat

1: 1000-1: 2000

lung, brain, kidney

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine-specific kinase.

Images

IHC analysis of S100A10(GB150109).
Sample: Human lung, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

IHC analysis of S100A10(GB150109).
Sample: Mouse brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

IF analysis of S100A10(GB150109)red.
Sample: Mouse kidney, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.