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Product Information
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Protein full name |
Trans-acting T-cell-specific transcription factor GATA-3 |
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Synonyms |
GATA3, GATA-binding factor 3, Trans acting T cell specific transcription factor GATA 3, GATA3_HUMAN, Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3 |
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Uniprot ID |
P23771 |
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Immunogen |
Recombinant protein corresponding to Human GATA3 |
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Isotype |
IgG1 |
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Clone Number |
SB433 |
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Predicted MW./Observed MW. |
48 kDa / 48 kDa |
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Purity |
Affinity purification |
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Subcellular location |
Nucleus |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human, Mouse, Rat | 1: 500-1: 1000 | SH-SY5Y, EL4, thymus |
| IHC/IF | Human, Mouse, Rat | 1: 1000-1: 2000 | placenta, skin, kidney |
Background
Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses. Positively regulates ASB2 expression.Coordinates macrophage transcriptional activation and UCP2-dependent metabolic reprogramming in response to IL33. Upon tissue injury, acts downstream of IL33 signaling to drive differentiation of inflammation-resolving alternatively activated macrophages.
Images
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WB analysis of GATA3(GB150082). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 5000, RT, 1h. |
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WB analysis of GATA3(GB150082). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 5000, RT, 1h. |
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IHC analysis of GATA3(GB150082). Sample: Human placenta, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min. |
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IHC analysis of GATA3(GB150082). Sample: Human placenta, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min. |
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IHC analysis of GATA3(GB150082). Sample: Mouse breast, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min. |
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IHC analysis of GATA3(GB150082). Sample: Mouse skin, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min. |
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IHC analysis of GATA3(GB150082). Sample: Rat kidney, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min. |
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IHC analysis of GATA3(GB150082). Sample: Rat skin, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min. |
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IHC analysis of GATA3(GB150082). Sample: Rat placenta, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min. |
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IHC analysis of GATA3(GB150082). Sample: Human placenta, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min. |
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IHC analysis of GATA3(GB150082). Sample: Mouse skin, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min. |
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IHC analysis of GATA3(GB150082). Sample: Human breast cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min. |
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IHC analysis of GATA3(GB150082). Sample: Human breast cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min. |
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IHC analysis of GATA3(GB150082). Sample: Rat skin, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min. |
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IF analysis of GATA3(GB150082)red. Sample: Human placenta, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h. |
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IF analysis of GATA3(GB150082)red. Sample: Mouse placenta, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h. |
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IF analysis of GATA3(GB150082)red. Sample: Rat skin, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 1500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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