Français
English
Product Information
|
Protein full name |
Matrix metalloproteinase-9 |
|
Synonyms |
MMP9, CLG4B, GELB, MANDP2, MMP-9, 92 kDa type IV collagenase, 92 kDa gelatinase, gelatinase B, matrix metallopeptidase 9 |
|
Uniprot ID |
P41245 |
|
Immunogen |
Recombinant protein corresponding to Mouse MMP9 |
|
Isotype |
IgG |
|
Clone Number |
SB421 |
|
Predicted MW./Observed MW. |
81 kDa / 92 kDa |
|
Purity |
Affinity purification |
|
Subcellular location |
Secreted |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human, Mouse, Rat | 1: 500-1: 2000 | lung, lung+LPS, HeLa |
| IHC/IF | Human, Mouse, Rat | 1: 500-1: 1500 | lung, esophageal adenocarcinoma, lung injury |
Background
May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
Images
|
|
WB analysis of MMP9(GB150096). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
|
|
WB analysis of MMP9(GB150096). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
|
|
IHC analysis of MMP9(GB150096). Sample: Human esophageal squamous cell carcinoma, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min. |
|
|
IHC analysis of MMP9(GB150096). Sample: Human lung, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min. |
|
|
IHC analysis of MMP9(GB150096). Sample: Mouse fibrotic liver, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min. |
|
|
IHC analysis of MMP9(GB150096). Sample: Mouse fibrotic liver, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min. |
|
|
IHC analysis of MMP9(GB150096). Sample: Rat lung injury, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min. |
|
|
IHC analysis of MMP9(GB150096). Sample: Rat lung injury, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min. |
|
|
IF analysis of MMP9(GB150096)red. Sample: Human esophageal adenocarcinoma, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
|
|
IF analysis of MMP9(GB150096)red. Sample: Human esophageal adenocarcinoma, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
|
|
IF analysis of MMP9(GB150096)red. Sample: Human lung, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
|
|
IF analysis of MMP9(GB150096)red. Sample: Human lung, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
|
|
IF analysis of MMP9(GB150096)red. Sample: Mouse fibrotic liver, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
|
|
IF analysis of MMP9(GB150096)red. Sample: Mouse fibrotic liver, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
|
|
IF analysis of MMP9(GB150096)red. Sample: Rat fibrotic liver, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
|
|
IF analysis of MMP9(GB150096)red. Sample: Rat lung injury, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
- Paste the label on a flat surface on the package
- Make sure that both 1D and 2D barcodes are clearly visible
- Ensure that the label is smooth and isn’t creased or wrinkled
- Check for any tears, dents, holes or scratches
- Pack your product tightly, with the right size packaging
- Ensure both barcodes are on a flat surface of the package