Recombinant Anti-PCNA antibody (Mouse mAb)

Recombinant Anti-PCNA antibody (Mouse mAb)

Antibodies-Oncology
AO-03-GB15010-50
PCNA,ATLD2,proliferating cell nuclear antigen
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€299.00
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Product Information


Protein full name

Proliferating cell nuclear antigen

Synonyms

PCNA, ATLD2, proliferating cell nuclear antigen

Uniprot ID

P17918

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse PCNA

Isotype

IgG1, κ

Clone Number

SB275

Predicted MW./Observed MW.

29 kDa / 36 kDa

Purity

Affinity purification

Subcellular location

Nucleus



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 1000-1: 2000 testis, placenta, C2C12
IHC/IF Human, Mouse, Rat 1: 800-1: 1000 esophageal carcinoma, testis, lung cancer



Background

Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics.



Images

01xTkIqYrS8iQUS.jpg

 WB analysis of PCNA(GB15010).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 5000, RT, 1h.

02gDEnPgHc4d2fv.jpg

 IHC analysis of PCNA(GB15010).
Sample: Human colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.

03t1ZHURR5DRufs.jpg

 IHC analysis of PCNA(GB15010).
Sample: Human lung cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.

04gRRQMomIhBhjF.jpg

 IHC analysis of PCNA(GB15010).
Sample: Mouse spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.

05XmY5jVCnhsYGu.jpg

 IHC analysis of PCNA(GB15010).
Sample: Rat spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.

06H9iLJZQsIdxXO.jpg

 IHC analysis of PCNA(GB15010).
Sample: Rat spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.

07BYTYqReNgXege.jpg

 IHC analysis of PCNA(GB15010).
Sample: Rat testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.

085VifdjPfAgqTq.jpg

 IF analysis of PCNA(GB15010)red.
Sample: Human esophageal carcinoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h.

09tKnLD5XPxhdoB.jpg

 IF analysis of PCNA(GB15010)red.
Sample: Human lung cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h.

1086BOWILS9EBuA.jpg

 IF analysis of PCNA(GB15010)red.
Sample: Mouse testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h.

11EWqDwsr2JqItY.jpg

 IF analysis of PCNA(GB15010)red.
Sample: Rat testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


Antibodies-Oncology
: AO-03-GB15010-50
: 10 Items
Hurry! only 10 items left in stock.

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