Recombinant Anti-Placental Alkaline Phosphatase (PLAP) antibody (Mouse mAb)

Recombinant Anti-Placental Alkaline Phosphatase (PLAP) antibody (Mouse mAb)

Antibodies-Oncology
AO-03-GB152545-100
Alkaline phosphatase Regan isozyme,Placental alkaline phosphatase 1,PLAP-1,ALPP,PLAP,DOA1,PLA2P,PLAA,PLAP,Glycerophosphatase,Alkaline phosphatase,placental type
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Product Information


Protein full name

Alkaline phosphatase, placental type

Synonyms

Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1, ALPP, PLAP, DOA1, PLA2P, PLAA, PLAP, Glycerophosphatase

Uniprot ID

P05187

Immunogen

Human recombinant PLAP protein expressed in HEK293 cells

Isotype

IgG1, κ

Clone Number

SB233

Predicted MW./Observed MW.

58 kDa / 68 kDa

Purity

Affinity purification

Subcellular location

Cell membrane



Applications


Applications Species Dilution Positive Tissue
WB Human 1: 3000-1:5000 HeLa
IHC/IF Human 1: 250-1: 1000 seminoma, placenta



Background

Alkaline phosphatase that can hydrolyze various phosphate compounds.



Images

01Mq2C1MjchNNaS.jpg

 WB analysis of Placental Alkaline Phosphatase (PLAP)(GB152545).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 3000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 5000, RT, 1h.

02LlN0shUxw2E2i.jpg

 IHC analysis of Placental Alkaline Phosphatase (PLAP)(GB152545).
Sample: Human placenta, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.

03AIkhzFTcCZnbD.jpg

 IHC analysis of Placental Alkaline Phosphatase (PLAP)(GB152545).
Sample: Human placenta, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.

0470auc0ZEx7Cik.jpg

 IHC analysis of Placental Alkaline Phosphatase (PLAP)(GB152545).
Sample: Human placenta, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.

05ONGmTxeXo3CGA.jpg

 IHC analysis of Placental Alkaline Phosphatase (PLAP)(GB152545).
Sample: Human seminoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.

06I1AsWDt7ct4gW.jpg

 IHC analysis of Placental Alkaline Phosphatase (PLAP)(GB152545).
Sample: Human seminoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG (H+L), Ready to use(G1301), RT, 20min.

07WbeBcge7H9qw1.jpg

 IF analysis of Placental Alkaline Phosphatase (PLAP)(GB152545)red.
Sample: Human placenta, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h.

08LPwK1Jhv8THwf.jpg

 IF analysis of Placental Alkaline Phosphatase (PLAP)(GB152545)red.
Sample: Human placenta, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


Antibodies-Oncology
: AO-03-GB152545-100
: 10 Items
Hurry! only 10 items left in stock.

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