Recombinant Anti-Galectin 3 antibody (Rabbit mAb)

Recombinant Anti-Galectin 3 antibody (Rabbit mAb)

Antibodies-Oncology
AO-03-GB151145-100
LGALS3,CBP35,GAL3,GALBP,GALIG,L31,LGALS2,MAC2,lectin,Galactoside binding soluble 3,Galectin 3,Gal-3,35 kDa lectin,L-31,Galactose-specific lectin 3,IgE-binding protein,Galectin-3
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Product Information


Protein full name

Galectin-3

Synonyms

LGALS3, CBP35, GAL3, GALBP, GALIG, L31, LGALS2, MAC2, lectin, Galactoside binding soluble 3, Galectin 3, Gal-3, 35 kDa lectin, L-31, Galactose-specific lectin 3, IgE-binding protein

Uniprot ID

P16110

Immunogen

Recombinant protein corresponding to Mouse Galectin 3

Isotype

IgG, κ

Clone Number

SB220

Predicted MW./Observed MW.

28 kDa / 30 kDa

Purity

Affinity purification

Subcellular location

Cytoplasm, Nucleus, Secreted



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 500-1: 1000 HeLa, MCF7, embryo
IHC/IF Human, Mouse, Rat 1: 1000-1: 4000 Inflammatory model induced by lps(6h) colon, Inflammatory model induced by lps(6h) lung, tonsil



Background

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.



Images

01eaJC2WxKBIU6v.jpg

 WB analysis of Galectin 3(GB151145).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

026r26oB6h4FPXM.jpg

 IHC analysis of Galectin 3(GB151145).
Sample: Mouse Inflammatory model induced by lps(24h)lung, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

037gYX2tpfmjq0z.jpg

 IHC analysis of Galectin 3(GB151145).
Sample: Rat Inflammatory model induced by lps(6h) lung, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

04A2fVox4Hqw6tx.jpg

 IHC analysis of Galectin 3(GB151145).
Sample: Mouse Inflammatory model induced by lps(6h) colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 4000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

05MTlHvesaTc7Ig.jpg

 IHC analysis of Galectin 3(GB151145).
Sample: Rat Inflammatory model induced by lps(6h) colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 4000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

06zT9IWTnsx1ebf.jpg

 IF analysis of Galectin 3(GB151145)red.
Sample: Human tonsil, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

07uKTYpVXl6JHxx.jpg

 IF analysis of Galectin 3(GB151145)red.
Sample: Mouse Inflammatory model induced by lps(24h) lung, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

08vc6QVpJUK5WWO.jpg

 IF analysis of Galectin 3(GB151145)red.
Sample: Rat Inflammatory model induced by lps(6h) lung, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


Antibodies-Oncology
: AO-03-GB151145-100
: 10 Items
Hurry! only 10 items left in stock.

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