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Recombinant Anti-Carbonic Anhydrase 9/CA9 antibody (Rabbit mAb)
AO-03-GB153899-50
Product Information
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Protein full name |
Carbonic anhydrase 9 |
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Synonyms |
CA IX, CA9, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, carbonic anhydrase IX, G250, Membrane antigen MN, MN, P54/58N, pMW1, RCC associated antigen G250 |
|
Uniprot ID |
Q16790 |
|
Immunogen |
Recombinant protein corresponding to Human Carbonic anhydrase9 |
|
Isotype |
IgG, κ |
|
Clone Number |
SB26 |
|
Purity |
Affinity purification |
|
Subcellular location |
Cell Membrane, Nucleus, Cell projection |
Applications
| Applications | Species | Dilution | Positive Tissue |
| IHC/IF | Human | 1: 500-1: 1000 | gastric carcinoma, colon cancer |
Background
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known.
Images
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IHC analysis of Carbonic Anhydrase 9/CA9(GB153899). Sample: Human colon cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
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IHC analysis of Carbonic Anhydrase 9/CA9(GB153899). Sample: Human gastric carcinoma, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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