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Product Information
|
Protein full name |
Napsin-A |
|
Synonyms |
Asp 4, ASP4, Aspartyl protease 4, KAP, Kdap, NAP1, NAPA, NAPSA, Napsin 1, Napsin A, napsin A aspartic peptidase, Pronapsin A, SNAPA, TA01/TA02, napsin A aspartic peptidase |
|
Uniprot ID |
O96009 |
|
Immunogen |
Recombinant protein corresponding to Human NAPSIN-A |
|
Isotype |
IgG1, κ |
|
Clone Number |
SB115 |
|
Purity |
Affinity purification |
|
Subcellular location |
Secreted |
Applications
| Applications | Species | Dilution | Positive Tissue |
| IHC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | lung |
Background
Napsin A is an aspartic proteinase that is expressed in normal lung and kidney. In the lung, napsin A is expressed by type II pneumocytes and alveolar macrophages, where it plays a role in processing surfactant protein B. Napsin A is expressed in lung adenocarcinomas, where it can be used to identify primary and metastatic lesions with greater sensitivity compared to TTF-1. Napsin A expression has also been described in other types of cancer, such as kidney and thyroid cancer.
Images
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IHC analysis of NAPSIN A(GB151011). Sample: Human lung, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. |
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IHC analysis of NAPSIN A(GB151011). Sample: Mouse lung, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. |
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IHC analysis of NAPSIN A(GB151011). Sample: Rat lung, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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