Recombinant Anti-S100A4 antibody (Rabbit mAb)

Protein full name

Protein S100-A4

Synonyms

S100A4, 18A2, 42A, CAPL, FSP1, MTS1, P9KA, PEL98, S100 calcium binding protein A4

Immunogen

Recombinant protein corresponding to Mouse S100A4

Uniprot ID

P26447, P07091, P05942

Isotype

IgG, κ

Clone Number

SB100

Purity

Affinity purification

Subcellular location

Secreted, Nucleus

Predicted MW./Observed MW.

12 kDa / 12 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 2000

HeLa, A549, C2C12,

IHC/IF

Human, Mouse, Rat

1: 800-1: 1600

spleen, colon, kidney, endometrial cancer

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis.

Images

WB analysis of S100A4(GB15398).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

IHC analysis of S100A4(GB15398).
Sample: Rat colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1200, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

IHC analysis of S100A4(GB15398).
Sample: Mouse spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1200, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.