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Product Information
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Protein full name |
ADAMTS-like protein 1 |
|
Synonyms |
Punctin-1, Punctin, Thrombospondin, C9orf94, ADAMTSL-1 |
|
Uniprot ID |
Q8BLI0 |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse ADAMTSL1 |
|
Isotype |
IgG |
|
Predicted MW./Observed MW. |
58 kDa / 80 kDa |
|
Purity |
Affinity purification |
|
Subcellular location |
Secreted |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human | 1: 300-1: 600 | U-251 MG, A431 |
| IHC/IF | Human | 1: 500-1: 1500 | esophagus cancer, tonsil |
Background
This gene encodes a secreted protein and member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) family. This protein lacks the metalloproteinase and disintegrin-like domains, which are typical of the ADAMTS family, but contains other ADAMTS domains, including the thrombospondin type 1 motif. This protein may have important functions in the extracellular matrix. Alternative splicing results in multiple transcript variants encoding distinct proteins.
Images
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WB analysis of ADAMTSL1(GB115331). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
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IHC analysis of ADAMTSL1(GB115331). Sample: Human esophagus cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
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IHC analysis of ADAMTSL1(GB115331). Sample: Human tonsil, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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