Anti-ErbB2/HER2 Rabbit pAb

Protein full name

Receptor tyrosine-protein kinase erbB-2

Synonyms

CD340, ERBB2, HER 2, HER 2/neu, HER2, MLN 19, MLN19, NEU, NGL, p185, p185erbB2, Proto oncogene c ErbB 2, Proto oncogene Neu, TKR1, HER-2, erb-b2 receptor tyrosine kinase 2

Immunogen

Recombinant protein corresponding to Human Receptor tyrosine-protein kinase erbB-2

Uniprot ID

P04626

Isotype

IgG1, κ

Clone Number

4E12F10

Purity

Affinity purification

Subcellular location

Cell membrane, Early endosome, Cytoplasm, perinuclear region, Nucleus

Predicted MW. / Observed MW.

139 kDa / 185 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human

1: 500-1: 1000

SK-BR-3, SK-OV-3

IHC/IF

Human

1: 250-1: 1000

breast cancer

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.

Images

WB analysis of ErbB2/HER2(GB12327).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

IHC analysis of ErbB2/HER2(GB12327).
Sample: Human breast cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody:HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.

IHC analysis of ErbB2/HER2(GB12327).
Sample: Human breast cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.