Anti-Cardiac Troponin T Rabbit pAb

Anti-Cardiac Troponin T Rabbit pAb

Antibodies-Oncology
AO-03-GB113806-50
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€299.00
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Product Information


Protein full name

Troponin T, cardiac muscle

Synonyms

Cardiac muscle troponin T, CMH2, CMPD2, cTnT, RCM3, TNNT2, TnTC, troponin T type 2 (cardiac), Troponin T, cardiac muscle, LVNC6, troponin T2, cardiac type

Uniprot ID

P50752

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Cardiac Troponin T

Isotype

IgG

Predicted MW./Observed MW.

36 kDa / 40 kDa

Purity

Affinity purification

Subcellular location

myofibril, sarcomere, sarcoplasm, striated muscle thin filament



Applications


Applications Species Dilution Positive Tissue
WB Mouse, Rat 1: 500-1: 1000 heart, skeletal muscle
IHC/IF Mouse, Rat 1: 500-1: 1000 heart



Background

Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.



Images

01y9nh4htG3gl2n.jpg

 WB analysis of Cardiac Troponin T(GB113806).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

021K0FmlvNkCHkP.jpg

 IHC analysis of Cardiac Troponin T(GB113806).
Sample: Mouse heart, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03qQ6W7l5OwEJVw.jpg

 IHC analysis of Cardiac Troponin T(GB113806).
Sample: Mouse heart, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

04TarzStI2DcNBO.jpg

 IHC analysis of Cardiac Troponin T(GB113806).
Sample: Rat heart, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

05dkLV3Da5vZaBS.jpg

 IHC analysis of Cardiac Troponin T(GB113806).
Sample: Rat heart, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

06hC9ZdSfryl6f5.jpg

 IF analysis of Cardiac Troponin T(GB113806)red.
Sample: Rat heart, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


Antibodies-Oncology
: AO-03-GB113806-50
: 10 Items
Hurry! only 10 items left in stock.

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