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Product Information
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Protein full name |
Serologically defined colon cancer antigen 8 homolog |
|
Synonyms |
Antigen NY CO 8, BBS16, CCCAP, hCCCAP, HSPC085, NY CO 8, SDCCAG8 |
|
Uniprot ID |
Q80UF4 |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse SDCCAG8 |
|
Isotype |
IgG |
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Purity |
Affinity purification |
|
Subcellular location |
Cytoplasm, Cytoskeleton |
Applications
| Applications | Species | Dilution | Positive Tissue |
| IHC/IF | Human, Mouse, Rat | 1: 1000-1: 3000 | colon cancer, ovary, thymus gland |
Background
Plays a role in the establishment of cell polarity and epithelial lumen formation. Plays also an essential role in ciliogenesis and subsequent Hedgehog signaling pathway that requires the presence of intact primary cilia for pathway activation. Mechanistically, interacts with and mediates RABEP2 centrosomal localization which is critical for ciliogenesis.
Images
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IHC analysis of SDCCAG8(GB114950). Sample: Human colon cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
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IHC analysis of SDCCAG8(GB114950). Sample: Mouse ovary, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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