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Product Information
|
Protein full name |
Protein S100-B |
|
Synonyms |
NEF, S-100 protein beta chain, S-100 protein subunit beta, S100, S100 Beta, S100 calcium binding protein B, S100B, S100beta, S100-B, S100β |
|
Uniprot ID |
P04271 |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Human S100 beta |
|
Isotype |
IgG |
|
Predicted MW./Observed MW. |
11 kDa / 11 kDa |
|
Purity |
Affinity purification |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human, Rat | 1: 300-1: 600 | brain, cerebellum |
Background
S100B is glial-specific and is expressed primarily by astrocytes, but not all astrocytes express S100B. It has been shown that S100B is only expressed by a subtype of mature astrocytes that ensheath blood vessels and by NG2-expressing cells.This protein may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly.
Images
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WB analysis of S100 beta(GB114581). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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