Anti-S100 beta Rabbit pAb

Protein full name

Protein S100-B

Synonyms

NEF, S-100 protein beta chain, S-100 protein subunit beta, S100, S100 Beta, S100 calcium binding protein B, S100B, S100beta, S100-B, S100β

Immunogen

Recombinant protein corresponding to Human S100 S100 beta

Uniprot ID

P04271

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus, Cytoplasm, Secreted

Applications

Species

Dilution

Positive Tissue

IHC/IF

Human, Mouse, Rat, Monkey

1: 500-1: 1000

colon, glioblastoma multiforme, brain

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

S100B is glial-specific and is expressed primarily by astrocytes, but not all astrocytes express S100B. It has been shown that S100B is only expressed by a subtype of mature astrocytes that ensheath blood vessels and by NG2-expressing cells.This protein may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly.

Images

IHC analysis of S100 beta(GB113884).
Sample: Human colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

IHC analysis of S100 beta(GB113884).
Sample: Mouse brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

IHC analysis of S100 beta(GB113884).
Sample: Rat colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.