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Product Information
|
Protein full name |
Probable threonine protease PRSS50 |
|
Synonyms |
Cancer/testis antigen 20, CT20, PRSS50, Serine protease 50, Testis-specific protease-like protein 50, TSP50 |
|
Uniprot ID |
Q8BLH5 |
|
Immunogen |
Recombinant protein corresponding to Mouse TSP50 |
|
Isotype |
IgG |
|
Predicted MW./Observed MW. |
49 kDa / 45 kDa |
|
Purity |
Affinity purification |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Mouse, Rat | 1: 2000-1: 4000 | brain, kidney, testis |
Background
TSP50 (Testis-specific protease-like protein 50) is also named as PRSS50, CT20 and belongs to the peptidase S1 family. It can enhance the expression of NFKB target genes involved in cell proliferation, and promotes cell proliferation, at least partially, through activation of the NFKB signalling pathway. There are two protein bands of 37 kDa and 43 kDa to be detected in human testes that the the difference is most likely caused by posttranslational proteolytic cleavage. The full length protein has a signal peptide with 39 amino acids and two glycosylation sites.
Images
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WB analysis of TSP50(GB113646). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 4000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
|
|
WB analysis of TSP50(GB113646). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 4000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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