Français
English
Product Information
|
Protein full name |
Uroplakin-1a |
|
Synonyms |
Tetraspanin 21, Tspan 21, TSPAN21, UP1A, UPIA, UPK1A, UPKA, uroplakin 1A, Uroplakin Ia |
|
Uniprot ID |
Q9D132 |
|
Immunogen |
Recombinant protein corresponding to Mouse Uroplakin Ia |
|
Isotype |
IgG |
|
Predicted MW./Observed MW. |
29 kDa / 29 kDa |
|
Purity |
Affinity purification |
|
Subcellular location |
Cell membrane |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Rat | 1: 300-1: 600 | prostate |
| IHC/IF | Mouse, Rat | 1: 2000-1: 4000 | bladder |
Background
Uroplakins are a group of urothelial differentiation-related membrane proteins. They are components of the asymmetric unit membrane (AUM), which forms the apical plaques of mammalian urothelium and is believed to play a role in strengthening the urothelial apical surface thus preventing the cells from rupturing during bladder distention . Uroplakin-1a (UPK1A) belongs to the tetraspanin (TM4SF) family. UPK1A plays an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane.
Images
|
|
WB analysis of Uroplakin Ia(GB113288). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
|
|
IHC analysis of Uroplakin Ia(GB113288). Sample: Mouse bladder, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 3000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
|
|
IHC analysis of Uroplakin Ia(GB113288). Sample: Rat bladder, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 3000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
- Paste the label on a flat surface on the package
- Make sure that both 1D and 2D barcodes are clearly visible
- Ensure that the label is smooth and isn’t creased or wrinkled
- Check for any tears, dents, holes or scratches
- Pack your product tightly, with the right size packaging
- Ensure both barcodes are on a flat surface of the package