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Product Information
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Protein full name |
Protein S100-B |
|
Synonyms |
NEF, S-100 protein beta chain, S-100 protein subunit beta, S100, S100 Beta, S100 calcium binding protein B, S100B, S100beta, S100-B, S100β |
|
Uniprot ID |
P50114 |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse S100 beta |
|
Isotype |
IgG1, κ |
|
Clone Number |
27# |
|
Purity |
Affinity purification |
|
Subcellular location |
Nucleus, Cytoplasm, Secreted |
Applications
| Applications | Species | Dilution | Positive Tissue |
| IHC/IF | Human, Mouse, Rat | 1: 1000-1: 2000 | brain, glioma |
Background
S100B is glial-specific and is expressed primarily by astrocytes, but not all astrocytes express S100B. It has been shown that S100B is only expressed by a subtype of mature astrocytes that ensheath blood vessels and by NG2-expressing cells.This protein may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly.
Images
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IHC analysis of S100 beta(GB12359). Sample: Human glioma, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. |
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IHC analysis of S100 beta(GB12359). Sample: Mouse brain, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. |
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IHC analysis of S100 beta(GB12359). Sample: Rat brain, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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