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Product Information
|
Protein full name |
Heparanase |
|
Synonyms |
Endo-glucoronidase, Hpse, Hpa, HEP, Heparanase 50 kDa subunit |
|
Uniprot ID |
Q6YGZ1 |
|
Immunogen |
Recombinant protein corresponding to Mouse Heparanase 1 |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Subcellular location |
Nucleus, Secreted, Lysosome |
Applications
| Applications | Species | Dilution | Positive Tissue |
| IHC/IF | Rat | 1: 100-1: 600 | kidney |
Background
Heparanase is an endo-beta-D-glucuronidase, which degrades heparan sulfate side chains of heparan sulfate proteoglycans (HSPGs) in the extracellular matrix. Heparanase plays an important role in ECM degradation, facilitating the migration and extravasations of tumor cells and inflammatory leukocytes. Upon degradation, heparanase releases growth factors and cytokines that stimulate cell proliferation and chemotaxis. Heparanase is a heterodimer comprised of a 50 kDa subunit harboring the active site and an 8 kDa subunit.
Images
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IHC analysis of Heparanase 1(GB112373). Sample: Rat kidney, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 300, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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