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Product Information
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Protein full name |
Palmitoyltransferase ZDHHC9 |
|
Synonyms |
Zinc finger DHHC domain-containing protein 9, DHHC-9, Zdhhc9, DHHC9, CGI89, CXorf11, Zinc finger protein 379 |
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Uniprot ID |
P59268 |
|
Immunogen |
Recombinant protein corresponding to Mouse ZDHHC9 |
|
Isotype |
IgG |
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Predicted MW./Observed MW. |
41 kDa / 41 kDa |
|
Purity |
Affinity purification |
|
Subcellular location |
Endoplasmic reticulum, Golgi apparatus, Membrane |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Mouse, Rat | 1: 500-1: 1000 | heart, brain, cortex |
| IHC/IF | Mouse, Rat | 1: 700-1: 1400 | prefrontal lobe, skin |
Background
This gene encodes an integral membrane protein that is a member of the zinc finger DHHC domain-containing protein family. The encoded protein forms a complex with golgin subfamily A member 7 and functions as a palmitoyltransferase. This protein specifically palmitoylates HRAS and NRAS. Mutations in this gene are associated with X-linked mental retardation. Alternate splicing results in multiple transcript variants that encode the same protein.
Images
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WB analysis of ZDHHC9(GB112279). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
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IF analysis of ZDHHC9(GB112279)red. Sample: Mouse prefrontal lobe, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 700, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
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IF analysis of ZDHHC9(GB112279)red. Sample: Rat skin, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 700, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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