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Product Information
|
Protein full name |
Anterior gradient protein 3 |
|
Synonyms |
Agr3, AG3, Anterior gradient homolog 3, BCMP11, hAG 3, PDIA18, Protein disulfide isomerase family A member 18 |
|
Uniprot ID |
Q8R3W7 |
|
Immunogen |
Recombinant protein corresponding to Mouse AG-3 |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Subcellular location |
Cytoplasm |
Applications
| Applications | Species | Dilution | Positive Tissue |
| IHC/IF | Human | 1: 600-1: 1500 | breast cancer, uterus |
Background
AGR3 (Anterior Gradient 3) protein, also known as AG3 (hAG-3, HAG3 in human), or BCMP11, is a secreted cytoplasmic protein which is involved in metastasis induction and p53 tumour supressor inhibition. It may serve as molecular marker and potential therapeutic target for hormone-responsive breast tumours. Its Xenopus homolog is associated with anteroposterior fate determination during early development.
Images
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IF analysis of AG-3(GB111744)red. Sample: Human breast cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 800, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
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IF analysis of AG-3(GB111744)red. Sample: Human uterus, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 800, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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