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Product Information
|
Protein full name |
Programmed cell death 1 ligand 1 |
|
Synonyms |
CD274, B7-H, B7H1, PDL-1, PDCD1L1, PDCD1LG1, PDL1, CD274 molecule, Programmed cell death 1 ligand 1 |
|
Uniprot ID |
Q9EP73 |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse PD-L1 |
|
Isotype |
IgG |
|
Predicted MW./Observed MW. |
33 kDa / 40-55 kDa |
|
Purity |
Affinity purification |
|
Subcellular location |
Cell membrane |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human, Mouse, Rat | 1: 600-1: 800 | skeletal muscle, placenta, lymph node, heart, liver cancer |
| IHC/IF | Human | 1: 500-1: 1000 | lung cancer |
Background
Plays a critical role in induction and maintenance of immune tolerance to self.As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response.Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10).Can also act as a transcription coactivator: in response to hypoxia, translocates into the nucleus via its interaction with phosphorylated STAT3 and promotes transcription of GSDMC, leading to pyroptosis.The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival.The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function.
Images
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WB analysis of PD-L1(GB11339A). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
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|
WB analysis of PD-L1(GB11339A). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 600, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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