Anti-Galectin 1 Rabbit pAb

Anti-Galectin 1 Rabbit pAb

Antibodies-Oncology
AO-03-GB111038-100
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Product Information


Protein full name

Galectin-1

Synonyms

Gal-1, 14 kDa lectin, Beta-galactoside-binding lectin L-14-I, Galaptin, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, S-Lac lectin 1, Lgals1, Gbp

Uniprot ID

P16045

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Galectin 1

Isotype

IgG

Predicted MW./Observed MW.

15 kDa / 15 kDa

Purity

Affinity purification

Subcellular location

Secreted



Applications


ApplicationsSpeciesDilutionPositive Tissue
WBMouse1: 500-1: 800heart, spleen, kidney
IHC/IFMouse1: 500-1: 2000brain, heart



Background


Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis. Plays a negative role in Th17 cell differentiation via activation of the receptor CD69.



Images

01U8ANxqi1NrZuZ.jpg                        

WB analysis of Galectin 1(GB111038).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02yexhnHX8PzgGk.jpg                        

WB analysis of Galectin 1(GB111038).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

039mROhp7sNamv6.jpg                        

IHC analysis of Galectin 1(GB111038).
Sample: Mouse heart, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer  (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

04lEb4XDqByBkPX.jpg                        

IHC analysis of Galectin 1(GB111038).
Sample: Mouse brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer  (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.



Storage


Storage            Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer            PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


Antibodies-Oncology
: AO-03-GB111038-100
: 10 Items
Hurry! only 10 items left in stock.

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