Anti-MMP2 Rabbit pAb

Protein full name

72 kDa type IV collagenase

Synonyms

MMP2, CLG4, CLG4A, MMP-2, MMP-II, MONA, TBE-1, matrix metallopeptidase 2

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse MMP2

Uniprot ID

P33434

Isotype

IgG

Purity

Affinity purification

Subcellular location

Secreted, Membrane, Nucleus, Cytoplasm

Predicted MW./Observed MW.

74 kDa / 74 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

lung

IHC/IF

Human, Mouse, Rat, Monkey, Pig

1: 1000-1: 2000

breast cancer, colon cancer, liver

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix(ECM) in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactiveproproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response.

Images

WB analysis of MMP2(GB11130).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

IHC analysis of MMP2(GB11130).
Sample: Human colon cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

IF analysis of MMP2(GB11130)red.
Sample: Human prostatic cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.