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Product Information
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Protein full name |
Alkaline phosphatase, tissue-nonspecific isozyme |
|
Synonyms |
ALPL, AP-TNAP, APTNAP, HOPS, TNAP, TNSALP, Alkaline phosphatase liver/bone/kidney, TNALP |
|
Uniprot ID |
P09242 |
|
Immunogen |
Recombinant protein corresponding to Mouse Alkaline Phosphatase |
|
Isotype |
IgG |
|
Predicted MW./Observed MW. |
57 kDa / 57 kDa |
|
Purity |
Affinity purification |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human, Mouse, Rat | 1: 500-1: 1000 | liver, kidney , cartilage |
Background
Intestinal, placental, placental-like, and liver/bone/kidney (tissue-nonspecific) all belong to alkaline phosphatases. the tissue-nonspecific form is located on chromosome 1. The product of this gene is a membrane-bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. This enzyme has been linked directly to hypophosphatasia disorder characterized by hypercalcemia and skeletal defects.
Images
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WB analysis of Alkaline Phosphatase(GB11528). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
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WB analysis of Alkaline Phosphatase(GB11528). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
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WB analysis of Alkaline Phosphatase(GB11528). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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