Anti-Cardiac Troponin I Rabbit pAb

Anti-Cardiac Troponin I Rabbit pAb

Antibodies-Oncology
AO-03-GB11393-100
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Product Information


Protein full name

Troponin I, cardiac muscle

Synonyms

Cardiac troponin I, TNNI3, TNNC1, cTnI, CMH7, cardiac muscle, TNNI3, Troponin I type 3 cardiac, cardiac type, CTnI, CMD1FF, CMD2A, RCM1, cTnI, troponin I3

Uniprot ID

P48787

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Cardiac Troponin I

Isotype

IgG

Predicted MW./Observed MW.

24 kDa / 28 kDa

Purity

Affinity purification



Applications


Applications Species Dilution Positive Tissue
WB Mouse, Rat 1: 500-1: 1000 heart



Background

Troponin I is a cardiac and skeletal muscle protein useful in the laboratory diagnosis of heart attack. Troponin I is a part of the troponin protein complex, where it binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place. Because of it, myosin cannot bind actin in relaxed muscle. When calcium binds to the troponin C it causes conformational changes which lead to dislocation of troponin I and finally tropomyosin leaves the binding site for myosin on actin leading to contraction of muscle.



Images

01NghqTa8wOFJbI.jpg

 WB analysis of Cardiac Troponin I(GB11393).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


Antibodies-Oncology
: AO-03-GB11393-100
: 10 Items
Hurry! only 10 items left in stock.

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