Anti-SPARC Rabbit pAb

Anti-SPARC Rabbit pAb

Antibodies-Oncology
AO-03-GB11611-100
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Product Information


Protein full name

SPARC

Synonyms

Basement membrane protein 40, Cysteine rich protein, Osteonectin, Secreted acidic cystein rich glycoprotein, Secreted protein acidic and rich in cysteine, Secreted protein acidic cysteine rich, BM 40, ON, OI17

Uniprot ID

P07214

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse SPARC

Isotype

IgG

Predicted MW./Observed MW.

35 kDa / 40 kDa

Purity

Affinity purification



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 500-1: 1000 skin, brain, testis



Background

SPARC (Secreted protein acidic and rich in cysteine) also known as Osteonectin (ON) and basement-membrane protein 40 (BM-40), is a glycoprotein in the bone that binds calcium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. Osteonectin also shows affinity for collagen in addition to bone mineral calcium. A correlation between osteonectin overexpression and ampullary cancers and chronic pancreatitis has been found.



Images

01gMByn7LXK3Nq6.jpg

 WB analysis of SPARC(GB11611).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


Antibodies-Oncology
: AO-03-GB11611-100
: 10 Items
Hurry! only 10 items left in stock.

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