Recombinant Anti-Phospho-ERK1(T202/Y204)+ERK2(T185/Y187) antibody (Rabbit mAb)

Protein full name

Mitogen-activated protein kinase 1

Synonyms

MAPK3, ERK-1, ERK1, ERT2, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, p44-ERK1, p44-MAPK, mitogen-activated protein kinase 3; MAPK1, ERK, ERK-2, ERK2, ERT1, MAPK2, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk, p42-MAPK

Immunogen

KLH conjugated Synthetic phosphopeptide corresponding to Human ERK1(T202/Y204)+ERK2 (T185/Y187)

Uniprot ID

P28482

Isotype

IgG

Clone Number

SB726

Purity

Affinity purification

Predicted MW./Observed MW.

41 kDa,43 kDa / 42 kDa,44 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 2000

UV treated NIH/3T3, UV treated HT-29

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Mitogen-activated protein kinase 3 is an enzyme that in humans is encoded by the MAPK3 gene. The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets.

Images

WB analysis of Phospho-ERK1(T202/Y204)+ERK2(T185/Y187)(GB150228).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.