Recombinant Anti-Phospho-PI3K p85 (Y467)+PI3K p55 (Y199) antibody (Rabbit mAb)

Protein full name

Phosphatidylinositol 3-kinase regulatory subunit alpha

Synonyms

PIK3R1, GRB1, Phosphatidylinositol 3-kinase regulatory subunit alpha, p85, pi3k p85, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha,Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha (PI3-kinase subunit p85-alpha, PtdIns-3-kinase regulatory subunit p85-alpha),p55,pi3k p55,P55G,PIK3R3,Phosphatidylinositol 3-kinase regulatory subunit gamma,PI3-kinase regulatory subunit gamma, PI3K regulatory subunit gamma, PtdIns-3-kinase regulatory subunit gamma

Immunogen

KLH conjugated Synthetic phosphopeptide corresponding to Human PI3R1

Uniprot ID

P27986

Isotype

IgG

Clone Number

SB684

Purity

Affinity purification

Predicted MW./Observed MW.

84 kDa / 55/85 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

pervanadate treated NIH/3T3

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling.Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement.

Images

WB analysis of Phospho-PI3K p85 (Y467)+PI3K p55 (Y199)(GB150210).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.