Anti-Phospho-H2A.X (S139) Rabbit pAb
   

Anti-Phospho-H2A.X (S139) Rabbit pAb

Antibodies-Oncology
AO-03-GB111841-100
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Product Information

Protein full name

Histone H2AX

Synonyms

H2a/x, Histone H2A.X, H2AFX, H2AX, H2A histone family member X, γ-H2AX, p-H2AX, p-h2ax

Immunogen

KLH conjugated Synthetic phosphopeptide corresponding to Human gamma H2A.X (phospho S139)

Uniprot ID

P16104

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus

Predicted MW. / Observed MW.

15 kDa / 15 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 100-1: 500

A549, L929, PC12

IHC/IF

Human, Mouse, Rat

1: 100-1: 500

spleen, testis

ICC/IF

Human, Mouse, Rat

1: 100-1: 500

UV treated A549 cells, UV treated L929 cells, UV treated PC-12 cells

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Histone H2A.X belongs to the histone H2A family, which is synthesized in G1 and S phase. It is involved in nucleosomal organization of chromatin together with other histone proteins, and is specially important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 (to form gamma-H2AFX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, which promotes DNA repair and maintains genomic stability.

Images

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WB analysis of Phospho-H2A.X (S139)(GB111841).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 100, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

IHC analysis of Phospho-H2A.X (S139)(GB111841).
Sample: Mouse spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Primary antibody: 1: 250, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

ICC analysis of Phospho- H2A.X (S139)(GB111841)red.
Sample: Left image untreated A549 cells, right image UV treated with 30min A549 cells, 4% PFA (G1101) 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 200, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

For Research Use Only!                                                  Ver. No.: V3.2-20250923


Antibodies-Oncology
: AO-03-GB111841-100
: 10 Items
Hurry! only 10 items left in stock.

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