Recombinant Anti-Phospho-mTOR(S2448) antibody (Rabbit mAb)

Recombinant Anti-Phospho-mTOR(S2448) antibody (Rabbit mAb)

Antibodies-Oncology
AO-03-GB150078-100
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Product Information


Protein full name

Serine/threonine-protein kinase mTOR

Synonyms

FK506-binding protein 12-rapamycin complex-associated protein 1, Mammalian target of rapamycin, Rapamycin target protein 1, FRAP, FRAP1, FRAP2, RAFT1, RAPT1, mTOR, P-MTOR

Uniprot ID

P42345

Immunogen

KLH conjugated Synthetic phosphopeptide corresponding to Human mTOR(S2448)

Isotype

IgG, κ

Clone Number

SB424

Predicted MW./Observed MW.

289 kDa / 289 kDa

Purity

Affinity purification



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 1000-1: 2000 Hela with Calyculin A



Background

MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. MTOR has a calculated molecular mass of 289 kDa, and always can be detected at about 250 kDa due to some modifications.



Images

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 WB analysis of Phospho-mTOR(S2448)(GB150078).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


Antibodies-Oncology
: AO-03-GB150078-100
: 10 Items
Hurry! only 10 items left in stock.

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