Anti-Phospho-S6 Ribosomal Protein (S235/S236) Rabbit pAb

Protein full name

Small ribosomal subunit protein eS6

Synonyms

40S ribosomal protein S6, Cell and organelle markers, Phosphoprotein NP33, Phospho-RPS6 (Ser235/236), Phospho-S6 Ribosomal Protein (Ser235/236), ribosomal protein S6, Ribosome Marker, RPS6, S6 Ribosomal Protein, p-S6RP, p-RPS6

Immunogen

KLH conjugated Synthetic phosphopeptide corresponding to Human RPS6 (S235/S236)

Uniprot ID

P62753

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus, Cytoplasm

Predicted MW. / Observed MW.

29 kDa / 32 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 2000-1: 3000

HeLa,NIH/3T3

IHC/IF

Human, Mouse, Rat

1: 700-1: 1400

endometrial cancer, ovarian cancer

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.

Images

WB analysis of S6 Ribosomal Protein (S235/S236)(GB115635).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 2000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

IHC analysis of S6 Ribosomal Protein (S235/S236)(GB115635).
Sample: Human endometrial Cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 700, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

IHC analysis of S6 Ribosomal Protein (S235/S236)(GB115635).
Sample: Mouse spleen, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 700, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.