Recombinant Anti-Phospho-AKT (S473) antibody (Rabbit mAb)

Protein full name

RAC-beta serine/threonine-protein kinase， RAC-gamma serine/threonine-protein kinase， RAC-alpha serine/threonine-protein kinase

Synonyms

AKT, AKT (pan), AKT pan, AKT1, AKT1/2/3, pan AKT, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha, total AKT, p-AKT,p-AKT1/2/3, p-akt, p-akt1/2/3

Immunogen

KLH conjugated Synthetic phosphopeptide corresponding to Human AKT(S473)

Uniprot ID

P31751, Q9Y243, P31749

Isotype

IgG, κ

Clone Number

SB96

Purity

Affinity purification

Predicted MW. / Observed MW.

56 kDa / 60 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 1000-1: 2000

HeLa,NIH3T3,C6

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

An important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt, and RSK.These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr.Akt plays a central role in mediating critical cellular responses including cell growth and survival, angiogenesis, and transcriptional regulation.While a number of Akt substrates are known (such as GSK-3, Bad, and caspase-9) many important substrates await discovery.

Images

WB analysis of Phospho-AKT (S473)(GB150002).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.