Anti-Phospho-MEK1/2 (S218/S222) Rabbit pAb

Protein full name

Dual specificity mitogen-activated protein kinase kinase 1/2

Synonyms

MAP2K1, MAP2K2, MEK1, PRKMK1，MEK2, MKK2, PRKMK2, p-MEK, p-MEK1, p-MEK2, p-MEK1/2, p-mek, p-mek1, p-mek1/2

Immunogen

KLH conjugated Synthetic phosphopeptide corresponding to Human MEK1/2（S218/S222)

Uniprot ID

Q02750,P36507

Isotype

IgG

Purity

Affinity purification

Predicted MW. / Observed MW.

43/41（MEK1）44(MEK2) kDa / 45 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 100-1: 300

HeLa, NIH3T3, C6

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor as well as interleukin (IL)-10 receptor. Directly phosphorylates STAT but also activates STAT signaling through the transactivation of other JAK kinases associated with signaling receptors.

Images

WB analysis of Phospho-MEK1/2 (S218/S222)(GB115603).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

WB analysis of Phospho-MEK1/2 (S218/S222)(GB115603).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.