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Product Information
| Protein full name | Insulin receptor substrate 1 |
| Synonyms | HIRS 1, IRS 1, IRS1, IRS-1, P-IRS1 |
| Uniprot ID | P35568 |
| Immunogen | KLH conjugated Synthetic phosphopeptide corresponding to Human IRS1(S307） |
| Isotype | IgG |
| Predicted MW./Observed MW. | 132 kDa / 180 kDa |
| Purity | Affinity purification |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human | 1: 300-1: 600 | 293 |
Background
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).
Images
| | WB analysis of Phospho-IRS1 (Ser307)(GB115456). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 300, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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