Anti-Phospho-CREB(S133) Rabbit pAb

Protein full name

Cyclic AMP-responsive element-binding protein 1

Synonyms

CREB-1, CAMP-responsive element-binding protein 1, CAMP responsive element binding protein 1, Active transcription factor CREB, P-CREB

Immunogen

KLH conjugated Synthetic phosphopeptide corresponding to Human CREB（S133）

Uniprot ID

P16220

Isotype

IgG

Purity

Affinity purification

Predicted MW./Observed MW.

35 kDa / 43 kDa

Applications

Species

Dilution

Positive Tissue

WB

Mouse, Rat

1: 300-1: 600

NIH/3T3, C6

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.

Images

WB analysis of Phospho-CREB(S133)(GB114684).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.