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Product Information
| Protein full name | Tyrosine-protein kinase JAK2 |
| Synonyms | AK2, JTK10, THCYT3, Janus kinase 2, MAX2, P-JAK2 |
| Uniprot ID | O60674 |
| Immunogen | KLH conjugated Synthetic phosphopeptide corresponding to Human JAK2(Tyr1007+Tyr1008) |
| Isotype | IgG |
| Predicted MW./Observed MW. | 131 kDa / 125 kDa |
| Purity | Affinity purification |
| Subcellular location | Cytoplasm, Membrane, Nucleus |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Human, Mouse, Rat | 1: 300-1: 600 | C6 |
| IHC/IF | Human | 1: 400-1: 800 | cervical carcinoma, lung adenocarcinoma |
Background
This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis.
Images
| | WB analysis of Phospho-JAK2(Y1007/Y1008)(GB114585). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 300, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
| | WB analysis of Phospho-JAK2(Y1007/Y1008)(GB114585). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 300, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h. |
| | IHC analysis of Phospho-JAK2(Y1007/Y1008)(GB114585). Sample: Human cervical carcinoma, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 650, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| | IHC analysis of Phospho-JAK2(Y1007/Y1008)(GB114585). Sample: Human lung adenocarcinoma non-phospho-blocking, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 650, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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