Anti-Iba1 Rabbit pAb, Primary Antibody

Anti-Iba1 Rabbit pAb, Primary Antibody

Antibodies Oncology
AO-03-GB113502-50

Anti-Iba1 Rabbit pAb

Volume : 50 µL
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€299.00
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I-D9 I-E6
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Product Information

Protein full name

Allograft inflammatory factor 1

Synonyms

AIF-1, IBA, IRT-1, IRT1, allograft, AIF-1, AIF1, G1, IBA1, IRT 1, Protein G1, Ionized calcium-binding adapter molecule 1, MRF1, Microglia response factor, BART 1, AIF1 protein

Immunogen

Recombinant protein corresponding to Mouse Iba1

Uniprot ID

O70200

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cell membrane, Cytoplasm, Cytoskeleton, Cell projection

Applications

IHC(PFA fixed)/IF(PFA fixed)

Human, Mouse, Rat, Monkey

1: 500-1: 1000

oligodendroglioma, brain, cerebellum

Background

AIF1, also named as G1, IBA1 or daintain/AIF-1, is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Despite a lack of detailed knowledge on the in vivo physiological functions of AIF-1, there is growing evidence that shows its aberrant expression contributes to the pathogenesis of many autoimmune diseases, including rheumatoid arthritis.  AIF1 is an actin-binding protein that enhances membrane ruffling and RAC activation. It enhances lymphocyte migration and the actin-bundling activity of LCP1.

Images

01 GB113502_20210816_IBA-1_1127 05_H oligodendroglioma d10000_IHC_20x.jpg

IHC analysis of IBA-1 (GB113502).

Sample: Human oligodendroglioma (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 1000, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

02 GB113502_20210816_IBA-1_1127 05_M brain d10000_IHC_20x.jpg

IHC analysis of IBA-1 (GB113502).

Sample: Mouse brain (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 1000, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

03 GB113502_20210816_IBA-1_1127 05_R brain d10000_IHC_20x.jpg

IHC analysis of IBA-1 (GB113502).

Sample: Rat brain (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 1000, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

04 GB113502_20210816_IBA-1_1127 05_R cerebellum d10000_IHC_20x.jpg

IHC analysis of IBA-1 (GB113502).

Sample: Rat cerebellum (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 1000, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

05 GB113502_20210826_IBA-1_112717112206 H oligodendroglioma d5000_IF_20x-1 2(1).jpg

IF analysis of IBA-1 (GB113502).

Sample: Human oligodendroglioma (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.

06 GB113502_20210826_IBA-1_112717112206 M brain d5000_IF_20x-1 2(1).jpg

IF analysis of IBA-1 (GB113502).

Sample: Mouse brain (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.

07 GB113502_20210826_IBA-1_112717112206 R brain d5000_IF_20x-1 2(1).jpg

IF analysis of IBA-1 (GB113502).

Sample: Rat brain (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.

08 GB113502_20210826_IBA-1_112717112206 M cerebellum d5000_IF_20x-1 2(1).jpg

IF analysis of IBA-1 (GB113502).

Sample: Mouse cerebellum (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.

09 GB113502_20210826_IBA-1_112717112206 R cerebellum d5000_IF_20x-1 2(1).jpg

IF analysis of IBA-1 (GB113502).

Sample: Rat cerebellum (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.

10 GB113502_IBA-1_ H oligodendrocytoma d500_40.0x_IHC.png

IHC analysis of IBA-1 (GB113502).

Sample: Human oligodendroglioma, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L), Ready to use(G1302), RT, 20min

11 GB113502_IBA-1_H glioblastoma multiforme d500_40x_IHC.png

IHC analysis of IBA-1 (GB113502).

Sample: Human glioblastoma multiforme, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L), Ready to use(G1302), RT, 20min.

18 GB113502_Iba1_d500_Mk brain_40.0x_IHC.jpg

 IHC analysis of IBA-1 (GB113502).

Sample: Monkey brain (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 500, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

Storage

Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

: AO-03-GB113502-50
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Hurry! only 10 items left in stock.

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