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Recombinant Anti-CD3 antibody (Rabbit mAb), Primary Antibody
AO-03-GB150004-50
Product Information
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Protein full name |
T-cell surface glycoprotein CD3 epsilon chain |
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Synonyms |
CD3E, IMD18, T3E, TCRE, CD3e molecule |
|
Immunogen |
Recombinant protein corresponding to Human CD3 |
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Uniprot ID |
P07766 |
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Isotype |
IgG |
|
Purity |
Affinity purification |
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Subcellular location |
Cell membrane |
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Predicted MW. / Observed MW. |
23 kDa / 20-50 kDa |
Applications
|
WB |
Human, Mouse, Rat |
1: 1000-1: 2000 |
Jurket, thymus, Molt-4 |
|
IHC/IF |
Human, Mouse, Rat |
1: 1000-1: 2000 |
stomach, spleen |
Background
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.
Images
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WB analysis of CD3(GB150004). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
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IHC analysis of CD3(GB150004). Sample: Human stomach, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203)), 98℃, 20 min. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
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IHC analysis of CD3(GB150004). Sample: Human tonsil, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203)), 98℃, 20 min. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
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IHC analysis of CD3(GB150004). Sample: Mouse spleen, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203)), 98℃, 20 min. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
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IHC analysis of CD3(GB150004). Sample: Rat spleen, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203)), 98℃, 20 min. Primary antibody: 1: 2000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
: AO-03-GB150004-50
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