Anti-Dnmt1 Rabbit pAb, Primary Antibody

Name: Anti-Dnmt1 Rabbit pAb, Primary Antibody
Brand: Antibodies Oncology
SKU: AO-03-GB11328
Price: 399 EUR
Availability: InStock

Brand : Antibodies Oncology

Reference : AO-03-GB11328-50

Anti-Dnmt1 Rabbit pAb

Anti-Dnmt1 Rabbit pAb.pdf

Volume : 50 µL

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Product Information

Protein full name	DNA (cytosine-5)-methyltransferase 1
Synonyms	DNA (cytosine-5-)-methyltransferase 1,DNA methyltransferase 1, DNMT1, ADCADN, AIM, CXXC9, DNMT, HSN1E, MCMT, m.HsaI
Uniprot ID	P13864
Immunogen	KLH conjugated Synthetic peptide corresponding to Mouse Dnmt1
Isotype	IgG
Purity	Affinity purification
Subcellular location	Nucleus

Applications

Applications	Species	Dilution	Positive Tissue
IHC/IF	Human	1: 500-1: 2000	colon, kidney, lung

Background

Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2.

Images

	IHC analysis of Dnmt1(GB11328). Sample: Human colon, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
	IHC analysis of Dnmt1(GB11328). Sample: Human lung, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.
	IF analysis of Dnmt1(GB11328)red. Sample: Human stomach, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.
	IF analysis of Dnmt1(GB11328)red. Sample: Human thyroid gland, 4% PFA (G1101) 12-24h. Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

Storage

Storage	Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer	PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.