

Monoclonal Anti-acetylated Histone H4
Ref: AO-01-H4-2C2
Volume: 10, 25, 50 and 100 µl
Format: Crude ascites fluid
Clone: 3HH4-2C2
Immunogen: synthetic peptide: MSGRG(acetyl-K)GG(acetyl-K)GLG(acetyl-K)GGA(acetyl K)RHRKVLR-(C) corresponding to aa 1 to 24 of human histone H4. This sequence is conserved in a wide range of species.
Murine Isotype: IgG1, κ
Specificity: Acetylated histone H4 at positions K5, K8, K12 or K16.
Applications :
· ELISA
· Western blot
· Immunoprecipitation
· Immunofluorescence
Recommended dilutions: 1/500-1/5000
Storage: Store at -20°C (up to 3 years) Avoid repeat freezing and thawing cycles.
Does not contain any preservative
References:
Brand M, Moggs JG, Oulad-Abdelghani M, Lejeune F, Dilworth FJ, et al. (2001) UV damaged DNA-binding protein in the TFTC complex links DNA damage recognition to nucleosome acetylation. Embo J 20: 3187-3196.
Dilworth FJ, Fromental-Ramain C, Yamamoto K, Chambon P. ATP-driven chromatin remodeling activity and histone acetyltransferases act sequentially during transactivation by RAR/RXR In vitro. Mol Cell. 2000. 6(5):1049-58
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