Recombinant Anti-Bcl-2 antibody (Mouse mAb)

Protein full name

Apoptosis regulator Bcl-2

Synonyms

BCL2, Bcl-2, PPP1R50, B-cell CLL/lymphoma 2, apoptosis regulator

Immunogen

Recombinant protein corresponding to Human Bcl-2

Uniprot ID

P10415

Isotype

IgG1, κ

Clone Number

SB64

Purity

Affinity purification

Predicted MW./Observed MW.

26 kDa / 26 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 1000-1: 2000

Jurkat,THP-1,MCF7,spleen

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells . Regulates cell death by controlling the mitochondrial membrane permeability . Appears to function in a feedback loop system with caspases . Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) . Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function . May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release .

Images

WB analysis of Bcl-2(GB154830).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 5000, RT, 1h.