Recombinant Anti-TMS1/ASC antibody (Rabbit mAb)

Protein full name

Apoptosis-associated speck-like protein containing a CARD

Synonyms

ASC, CARD5, hASC, PYCARD, PYD and CARD domain containing, TMS, TMS 1, TMS1

Immunogen

Recombinant protein corresponding to Mouse TMS1/ASC

Uniprot ID

Q9EPB4

Isotype

IgG, κ

Clone Number

SB363

Purity

Affinity purification

Subcellular location

Cytoplasm,Inflammasome, Endoplasmic reticulum, Mitochondrion, Nucleus

Predicted MW. / Observed MW.

22 kDa / 22-25 kDa

Applications

Species

Dilution

Positive Tissue

WB

Mouse

1: 1000-1: 2000

spleen, colon

IHC/IF

Mouse

1: 800-1: 1600

small intestine, colon, spleen

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

PYCARD , also known as ASC or TMS1, is a 195 amino acid protein containing an N-terminal Pyrin-like domain (PYD) and an 87 residue C-terminal Caspase-associated recruitment domain (CARD). It promotes caspase-mediated apoptosis which is mediated predominantly through the activation of caspase-9.

Images

WB analysis of TMS1/ASC(GB153966).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

IHC analysis of TMS1/ASC(GB153966).
Sample: Mouse Inflammatory model induced by lps(6h) small intestine, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

IF analysis of TMS1/ASC(GB153966)red.
Sample: Mouse Inflammatory model induced by lps(24h) colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 800, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.