Recombinant Anti-Caspase-7 antibody (Mouse mAb)

Recombinant Anti-Caspase-7 antibody (Mouse mAb)

Antibodies Oncology
AO-03-GB15540-50
Apoptotic protease Mch 3,CMH-1,MCH3,15540,ICE like apoptotic protease 3,ICE-LAP3,CASP-7,Caspase7,CASP7,Caspase 7,GB15540,casepase,Caspase-7,capase
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Product Information


Protein full name

Caspase-7

Synonyms

Apoptotic protease Mch 3, CASP-7, CASP7, Caspase 7, Caspase7, CMH-1, ICE-LAP3, ICE like apoptotic protease 3, MCH3

Uniprot ID

P55210

Immunogen

Recombinant protein corresponding to Human Caspase-7

Isotype

IgG1, κ

Clone Number

SB66

Predicted MW./Observed MW.

34 kDa / 20/30/35 kDa

Purity

Affinity purification

Subcellular location

Cytoplasm, Nucleus, Secreted



Applications


Applications Species Dilution Positive Tissue
WB Human 1: 500-1: 2000 Jurkat
IHC/IF Human, Mouse, Rat 1: 1000-1: 3000 colon, small intestine



Background

Caspase 7(CASP7), like caspases 3 and 6, contains a short prodomain and, upon apoptotic induction, the 35 kDa proform is converted into a 30 kDa intermediate or preactive form which is further processed into two active subunits consisting of the p20 or large (18 kDa) subunit and the p10 or small (11 kDa) subunit and it is present in the brain, which is up-regulated and activated after traumatic injury. The protein is involved in the activation cascade of caspases responsible for apoptosis execution.



Images

01C8ml9t3gw7pe0.jpg

 WB analysis of Caspase-7(GB15540).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 5000, RT, 1h.

02IfJIi7ilxxrzO.jpg

 IHC analysis of Caspase-7(GB15540).
Sample: Human colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.

03EUo6Ay6EjmysF.jpg

 IHC analysis of Caspase-7(GB15540).
Sample: Mouse small intestine, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.

04aVaakGEGtllrF.jpg

 IHC analysis of Caspase-7(GB15540).
Sample: Rat small intestine, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB15540-50
: 10 Items
Hurry! only 10 items left in stock.

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