Anti-ANP32C/PP32R1 Rabbit pAb
   

Anti-ANP32C/PP32R1 Rabbit pAb

Antibodies Oncology
AO-03-GB114550-50
PP32R1,ANP-32C,Tumorigenic protein pp32r1,GB114550,114550,pp32 related 1,ANP32C,PP 32R 1,PP-32R-1,ANP 32C,ANP32C/PP32R1,Acidic leucine-rich nuclear phosphoprotein 32 family member C
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Product Information


Protein full name

Putative uncharacterized protein ANP32CP

Synonyms

PP32R1, pp32 related 1, ANP32C, Tumorigenic protein pp32r1

Uniprot ID

O43423

Immunogen

Recombinant protein corresponding to Human ANP32C/PP32R1

Isotype

IgG

Predicted MW./Observed MW.

27 kDa / 28 kDa

Purity

Affinity purification

Subcellular location

Nucleus



Applications


Applications Species Dilution Positive Tissue
WB Human, Mouse, Rat 1: 500-1: 1000 prostate,lymph node
IHC/IF Human 1: 500-1: 1000 endometrial carcinoma, kidney



Background

The protein encoded by ANP32C is one of at least two proteins that are similar in amino acid sequence to PP32 and are part of the same acidic nuclear phosphoprotein gene family. However, unlike PP32, the encoded protein is tumorigenic. The tumor suppressor function of PP32 has been localized to a 25 amino acid region that is divergent between PP32 and the protein encoded by this gene.



Images

01nIoq0dvkcR1VB.jpg

 WB analysis of ANP32C/PP32R1(GB114550).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02cqTSLhO6y0zQU.jpg

 WB analysis of ANP32C/PP32R1(GB114550).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

03d5LhhywG2pqvA.jpg

 IHC analysis of ANP32C/PP32R1(GB114550).
Sample: Human endometrial carcinoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

04KLVBcUM7MrtWX.jpg

 IHC analysis of ANP32C/PP32R1(GB114550).
Sample: Human kidney, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98℃, 20 min.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

05RghcUgyovk5Lx.jpg

 IF analysis of ANP32C/PP32R1(GB114550)red.
Sample: Human esophageal carcinoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

06sdCc6iGgl7kQp.jpg

 IF analysis of ANP32C/PP32R1(GB114550)red.
Sample: Human kidney, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

07R16CvUEzJn0lC.jpg

 IF analysis of ANP32C/PP32R1(GB114550)red.
Sample: Human renal carcinoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98℃, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.



Storage


Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


: AO-03-GB114550-50
: 10 Items
Hurry! only 10 items left in stock.

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